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KMID : 0861120120160010071
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Korean Journal of Oriental Preventive Medicine
2012 Volume.16 No. 1 p.71 ~ p.80
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The anti-cancer effects of Extract (AE) on A549 cells - The role of Bcl-2 family protein on the AE-induced apoptosis -
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Nam ye-Seon
Cho Min-Kyung
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Abstract
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Objective: The aim of this study is to evaluate anti-cancer effects of Extract (AE) on human lung cancer A549 cells.
Method: The apoptotic activities and cell growth arrest activities of AE were measured using 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The molecules involved in apoptotic process were assessed by western blotting.
Result: Treatment of AE potently reduced cell viability in a dose-dependent manner in A549 cells. AE (100-500 ) resulted in apoptosis via activation of caspase 9 following PARP cleavage in a time-and dose-dependent manner. The levels of Bax and Bad levels were increased by AE with a concomitant decrease of Bcl-xL. In addition, AE at the low dose (30 ) significantly inhibited cell growth in the presence of serum.
Conclusion: AE has the potential as a therapeutic agent against lung cancer.
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KEYWORD
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Ampelopsis radix, Lung cancer, Apoptosis, Bcl-2 family
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